皖西白鹅换羽前后基因表达谱差异分析

doi:10.11843/j.issn.0366-6964.2013.07.006

摘要/Abstract

摘要：

旨在研究影响羽绒再生过程关键基因的表达调控。本试验选取6只100日龄皖西白鹅母鹅，于换羽前和换羽后1 d分别取其皮肤样，提取RNA，等量混合后比较换羽前后皖西白鹅差异表达基因。结果表明，Wnt信号通路中的wnt5a、wnt10a及Lipoprotein receptor-related protein 3 (LRP3)均表达下调，wnt受体基因frizzled 2 (FZD2)、FZD9、SFRP2表达上调，而FRZB、FZD8、FZD10表达下调；Noggin途径的NOG2和NOG均表达下调，BMP家族的BMPR1A和BMP1表达上调，BMP2、BAMPI、BMP2K及BMP7等基因均表达下调；涉及羽绒再生循环的其他相关基因如细胞周期相关蛋白基因Cyclin B3和Cyclin Y表达上调，而Cyclin D3和CDK3表达下调；皖西白鹅羽绒再生过程中还检测到类胰岛素生长因子IGF2、转化生长因子IGFα、白介素家族IL6ST等、肿瘤坏死因子TNFSF15等基因差异表达。皖西白鹅换羽1 d后引起Wnt经典途径和Noggin路径等多条信号通路上相关基因的差异表达。通过对皖西白鹅换羽前后基因表达差异分析，为进一步丰富禽类羽绒再生分子机理提供重要信息，羽绒再生过程关键调控基因的筛选将为鹅高产羽绒品系的选育提供依据。

Abstract:

The aim of this study was to exploit genes that play key roles during feather regeneration. In this experiment, 6 female Wanxi-white geese with 100-day-old were selected for skin samples before and 1 d after moulting. The 6 RNA samples were equally mixed for detecting the differentially expressed genes before and after moulting in Wanxi-white goose. The results showed that Wnt5a, Wnt10a and Lipoprotein receptor-related protein 3 (LRP3) genes in Wnt signaling pathway were down-regulated, and wnt receptors genes (frizzled 2 (FZD2), FZD9 and SFRP2) were up-regulated while FRZB, FZD8 and FZD10 were down-regulated 1 d after moulting in Wanxi-white goose. In the Noggin signaling pathway, NOG2 and NOG were down-regulated, and BMPR1A and BMP1 were up-regulated while BMP2, BAMPI, BMP2 and BMP7 were down-regulated 1 d after moulting. Cell cycle-related protein Cyclin B3 and Cyclin Y were up-regulated while Cyclin D3 and CDK3 were down-regulated. Other genes, such as IGF2, IGFα, IL6ST, TNFSF15, were also detected as differentially expressed genes 1 d after moulting in Wanxi-white goose. Many genes which participated in Wnt signaling pathway and Noggin signaling pathway, were detected in differential expression 1 d after moulting in Wanxi-white goose. The differentially expressed genes detected in this experiment would provide more information in the molecular regulation of downy feather regeneration. Genes screened from this experiment might be taken as key regulatory genes for breeding of goose with high-yield downy feather.

中图分类号:

S835
S813.3

陈兴勇，谢珊珊，周丽，姜润深，耿照玉. 皖西白鹅换羽前后基因表达谱差异分析[J]. 畜牧兽医学报, 2013, 44(7): 1030-1036.

CHEN Xing-yong, XIE Shan-shan, ZHOU Li, JIANG Run-shen, GENG Zhao-yu. Identification of Differentially Expressed Genes in Skin of Wanxi-white Goose during Regeneration of Downy Feather[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(7): 1030-1036.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/10.11843/j.issn.0366-6964.2013.07.006

https://www.xmsyxb.com/CN/Y2013/V44/I7/1030

参考文献

［1］SCHMIDT-ULLRICH R, PAUS R. Molecular principles of hair follicle induction and morphogenesis ［J］. Bioessays, 2005, 27:247-261.

［2］SUZUKI S, KATO T, TAKIMOTO H, et al. Localization of rat FGF-5 protein in skin macrophage-like cells and FGF-5S protein in hair follicle: possible involvement of two Fgf-5 gene products in hair growth cycle regulation ［J］. J Invest Dermatol, 1998, 111:963-972.

［3］PIERCE G F, YANAGIHARA D, KLOPCHIN K, et al. Stimulation of all epithelial elements during skin regeneration by keratinocyte growth factor ［J］. J Exp Med, 1994, 179:831-840.

［4］BOTCHKAREV V A, BOTCHKAREVA N V, NAKAMURA M, et al. Noggin is required for induction of the hair follicle growth phase in postnatal skin ［J］. FASEB J, 2001, 15:2205-2214.

［5］ITO M, YANG Z, ANDL T, et al. Wnt-dependent de novo hair follicle regeneration in adult mouse skin after wounding ［J］. Nature, 2007, 447:316-320.

［6］ZHANG Y, TOMANN P, ANDL T, et al. Reciprocal requirements for EDA/EDAR/NF-kappaB and Wnt/beta-catenin signaling pathways in hair follicle induction ［J］. Dev Cell, 2009, 17:49-61.

［7］FOITZIK K, LINDNER G, MUELLER R S, et al. Control of murine hair follicle regression (catagen) by TGF-beta1 in vivo ［J］. FASEB J, 2000, 14:752-760.

［8］SHAROV A A, SHAROVA T Y, MARDARYEV A N, et al. Bone morphogenetic protein signaling regulates the size of hair follicles and modulates the expression of cell cycle-associated genes ［J］. Proc Natl Acad Sci USA, 2006, 103:18166-18171.

［9］BOTCHKAREV V A, BOTCHKAREVA N V, SHAROV A A, et al. Modulation of BMP signaling by noggin is required for induction of the secondary (nontylotrich) hair follicles ［J］. J Invest Dermatol, 2002, 118:3-10.

［10］DASGUPTA R, FUCHS E. Multiple roles for activated LEF/TCF transcription complexes during hair follicle development and differentiation ［J］. Development, 1999, 126:4557-4568.

［11］CHEN X Y, BAI H C, LI L, et al. Follicle characteristics and follicle developmental related Wnt6 polymorphism in Chinese indigenous Wanxi-white goose ［J］. Mol Biol Rep, 2012, 39:9843-9848.

［12］MILLAR S E, WILLERT K, SALINAS P C, et al. WNT signaling in the control of hair growth and structure ［J］. Dev Biol, 1999, 207: 133-149.

［13］REDDY S, ANDL T, BAGASRA A, et al. Characterization of Wnt gene expression in developing and postnatal hair follicles and identification of Wnt5a as a target of Sonic hedgehog in hair follicle morphogenesis ［J］. Mech Dev, 2001, 107: 69-82.

［14］ANDREAS W, ROEL N. Mechanisms of Wnt signaling in development ［J］. Cell Dev Biol, 1998, 14:59-88.

［15］MOON R T, BROWN J D, TORRES M. WNTs modulate cell fate and behavior during vertebrate development ［J］. Trends Genet, 1997, 13:157-162.

［16］NIIDA A, HIROKO T, KASAI M, et al. DKK1, a negative regulator of Wnt signaling, is a target of the beta-catenin/ TCF pathway ［J］. Oncogene, 2004, 23:8520-8526.

［17］CHIANG C, SWAN R Z, GRACHTCHOUK M, et al. Essential role for Sonic hedgehog during hair follicle morphogenesis ［J］. Dev Biol, 1999, 205: 129.

［18］EROSHKIN F M, ERMAKOVA G V, BAYRAMOV A V, et al. Multiple noggins in vertebrate genome: cloning and expression of noggin2 and noggin4 in Xenopus laevis ［J］. Gene Expr Patterns, 2006, 6:180-186.

［19］YU M K, YUE Z C, WU P, et al. The developmental biology of feather follicles ［J］. Int J Dev Biol, 2004, 48:181-191.

［20］SCHMIDT-ULLRICH R, PAUS R. Molecular principles of hair follicle induction and morphogenesis ［J］. Bioessays, 2005, 27: 247-261.

［21］STENN K S, PAUS R. Controls of hair follicle cycling ［J］. Physiol Rev, 2001, 81: 449-494.

［22］MA L, LIU J, WU T, et al. Cyclic alopecia’ in Msx2 mutants: defects in hair cycling and hair shaft differentiation ［J］. Development, 2003, 130:379-389.

［23］ANDL T, AHN K, KAIRO A, et al. Epithelial Bmpr1a regulates differentiation and proliferation in postnatal hair follicles and is essential for tooth development ［J］. Development, 2004, 131:2257-2268.

［24］MILL P, MO R, FU H, et al. Sonic hedgehog-dependent activation of Gli2 is essential for embryonic hair follicle development ［J］. Genes Dev, 2003, 17:282-294.

[1]	马帅, 王燕, 庄新娟, 王文正, 赵茹茜. 母鹅日粮添加甜菜碱通过IGFs信号通路促进子代胸肌肌纤维肥大[J]. 畜牧兽医学报, 2023, 54(12): 5112-5124.
[2]	张力, 许加龙, 黄锦钰, 许子月, 雷昕诺, 卢会鹏, 朱睿, 孙伟翔, 曹海月, 王安平, 朱善元. 鹅骨骼肌卫星细胞的分离培养与鉴定[J]. 畜牧兽医学报, 2023, 54(10): 4186-4195.
[3]	卿恩华, 唐彬铖, 牛添, 王珺琦, 陈朝颜, 胡继伟, 何桦, 李亮, 王继文, 胡深强. 不同旱养方式对鹅睾丸及外生殖器组织形态学的影响[J]. 畜牧兽医学报, 2023, 54(7): 2872-2885.
[4]	袁岩聪, 何航, 刘安芳, 万堃, 章杰. 不同体重四川白鹅消化生理、免疫和肠道微生物的比较分析[J]. 畜牧兽医学报, 2023, 54(3): 1124-1134.
[5]	王猛, 李尚民, 沈丹, 韩国锋, 李延森, 窦新红, 李春梅. 饲粮不同比例青贮玉米对仔鹅生长性能、屠宰性能、肉品质及血清生化指标的影响[J]. 畜牧兽医学报, 2021, 52(12): 3501-3511.
[6]	张克山, 高广亮, 李琴, 赵献芝, 李静, 王启贵. NPFFR1基因对鹅卵泡颗粒细胞激素分泌和细胞凋亡的影响研究[J]. 畜牧兽医学报, 2021, 52(10): 2822-2831.
[7]	陈哲, 陈蓉, 闫乐艳, 陈佳彬, 于建宁. 鹅MyoG基因启动子活性及转录调控元件分析[J]. 畜牧兽医学报, 2021, 52(7): 1869-1879.
[8]	邓艳, 解广娟, 胡深强, 李亮, 王继文. miR-148a-3p靶向PPARγ基因抑制鹅颗粒细胞孕酮的合成[J]. 畜牧兽医学报, 2021, 52(6): 1571-1581.
[9]	王慧芳, 周光现, 孙永峰, 陈新企, 雷栩斌, 张锐毅, 贾汝敏, 赵志辉. 基于全基因组重测序技术的狮头鹅Indel标记分析[J]. 畜牧兽医学报, 2021, 52(3): 662-675.
[10]	欧阳宏佳, 孙敬帅, 江丹莉, 潘建秋, 黄运茂. 水禽StAR基因克隆、表达及其对睾丸发育的影响[J]. 畜牧兽医学报, 2020, 51(12): 3013-3022.
[11]	章杰, 熊子标, 李龙娇, 田旭, 揭晓蝶, 程雅婷, 万堃, 刘安芳, 向邦全, 何航. 不同体重鹅肝脏转录组的功能分析[J]. 畜牧兽医学报, 2020, 51(5): 987-996.
[12]	甘翔, 王继文, 李琴, 邓艳, 胡继伟, 李亮, 韩春春. 鹅成熟卵泡壁层组织动态发育特点-卵泡发育与分级的新视角[J]. 畜牧兽医学报, 2019, 50(8): 1607-1613.
[13]	刘同君, 赵盼, 赵敏孟, 刘龙, 崔恒宓, 龚道清, 耿拓宇. 内质网应激标记基因Grp78参与鹅肥肝免疫/炎症状态的调控[J]. 畜牧兽医学报, 2019, 50(4): 727-737.
[14]	王惠影, 郭保地, 何大乾, 龚绍明, 刘毅, 李光全, 徐琪, 陈国宏. 饲粮蛋氨酸对鹅再生羽绒品质、血清指标及基因mRNA表达量的影响[J]. 畜牧兽医学报, 2019, 50(2): 323-331.
[15]	周海柱, 高云航, 徐博, 陶大鹏, 滕战伟, 娄玉杰. 日粮纤维水平对鹅盲肠细菌多样性的影响[J]. 畜牧兽医学报, 2018, 49(3): 565-571.